PAST4FUTURE aims to generate and provide cells, culture systems, protocols for wide implementation of organotypic skin models to all researchers who are working on or with skin. Furthermore we aim to create a platform for researchers to interact, share expertise and experiences to move the field forward.
Once developed and available, resources will be posted here.
Project presentations:
Luca Meesters and Noa van de Brink presented their research that relates to the PAST4FUTURE project at the annual meeting of the European Society for Dermatological Research (ESDR) and at the satellite symposium of the European Epidermal Barrier Research Network (E2BRN), both in Amsterdam. Luca showed her work on new organotypic models for atopic dermatitis and psoriasis and Noa demonstrated an novel method for easy quantitative analysis of skin barrier function in organotypic skin models.
Their abstracts can be found in the abstract book, search for number 194 and 195!
PhD student Luca Meesters (RUMC) presented the PAST4FUTURE project with a poster at the PhD retreat for the RIMLS graduate school and RIMLS New Frontiers Symposium: Towards new epidermal cell sources and data-driven optimized organotypic skin models
At the annual NVED scientific meeting, Luca will present her work on the “Untangling the complex cytokine milieu in psoriasis and atopic dermatitis through in vitro epidermal models”
See here the poster from Luca
Cellular resources:
- Immortalized keratinocyte cell lines (N/TERT1; N/TERT-2G) developed by J. Rheinwald, Harvard University.
- Cells are available upon request but only for academic research purposes and not for commercial use.
Literature sources:
Our review and Research Techniques Made Simple articles on CRISPR-Cas9 technology in skin research published in the Journal of Investigative Dermatology:
CRISPR-Cas9‒Based Genomic Engineering in Keratinocytes: From Technology to Application
Research Techniques Made Simple: Delivery of the CRISPR/Cas9 Components into Epidermal Cells
Laboratory protocols:
Other relevant literature:
past4future.org 