Resources

PAST4FUTURE aims to generate and provide cells, culture systems, protocols for wide implementation of organotypic skin models to all researchers who are working on or with skin. Furthermore we aim to create a platform for researchers to interact, share expertise and experiences to move the field forward.

Once developed and available, resources will be posted here.

Project presentations:

PhD student Luca Meesters (RUMC) presented the PAST4FUTURE project with a poster at the PhD retreat for the RIMLS graduate school and RIMLS New Frontiers Symposium: Towards new epidermal cell sources and data-driven optimized organotypic skin models

At the annual NVED scientific meeting, Luca will present her work on the “Untangling the complex cytokine milieu in psoriasis and atopic dermatitis through in vitro epidermal models”

See here the poster from Luca

Cellular resources:

Literature sources:

Our review and Research Techniques Made Simple articles on CRISPR-Cas9 technology in skin research published in the Journal of Investigative Dermatology:

CRISPR-Cas9‒Based Genomic Engineering in Keratinocytes: From Technology to Application

Research Techniques Made Simple: Delivery of the CRISPR/Cas9 Components into Epidermal Cells

Laboratory protocols:

Organotypic 3D Skin Models: Human Epidermal Equivalent Cultures from Primary Keratinocytes and Immortalized Keratinocyte Cell Lines. Rikken G. et al. 2020

Pluripotent Stem Cell Differentiation Toward Functional Basal Stratified Epithelial Cells. Soares et al. 2020

Other relevant literature:

Perspective and Consensus Opinion: Good Practices for Using Organotypic Skin and Epidermal Equivalents in Experimental Dermatology Research. Van den Bogaard EH et al. 2021

Reconstructed human skin shows epidermal invagination towards integrated neopapillae indicating early hair follicle formation in vitro. Vahav et al. 2020

Recapitulation of Native Dermal Tissue in a Full-Thickness Human Skin Model Using Human Collagens. Mieremet et al. 2018

Models for cutaneous wound healing. Boekema et al. 2017

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